Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.Īpproximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 ☌), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). The ladder is supplied in gel loading buffer and is ready to use. The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. All markers are optimized for use with LI-COR imaging systems but can be used with other imagers. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). A protein marker (also called a protein molecular weight marker, a protein MW marker, or a protein ladder) is used to estimate the size of proteins resolved by gel electrophoresis. ladder, and the absence of additional intermediate bands at this height of the. The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. 1.5-2.5 μL per well for general Western transfering.īLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane. Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
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